WebNov 13, 2024 · HAP2/GCS1 is a protein identified in plants, protozoan parasites and other organisms that has an important role during membrane fusion in fertilization processes. ... The conserved plant sterility gene HAP2 functions after attachment of fusogenic membranes in Chlamydomonas and Plasmodium gametes. Genes Dev. 2008;22(8):1051–68. Article … WebJan 1, 2024 · Hydroxyproline-rich GP1 as an anchor for Chlamydomonas cell wall ... The screening experiment with pJPZ11_HAP2 did not result in any positive candidate, the outcome was confirmed by 50 mL cultures of the top candidate (Supp. Fig. S.3). The maximum RFU obtained during the screening in the 96 well plates for pJPZ11_HAP2 …
William J. Snell
WebJul 9, 2024 · The cellular fusion protein HAP2, which is structurally homologous to viral class II fusion proteins, drives gamete fusion across several eukaryotic kingdoms. Gamete … Web1 Introduction. Chlamydomonas is an excellent model system to study the regulation of cilia and flagella. All major structural components of cilia are conserved in this unicellular … daily pips hunter
Fusion surface structure, function, and dynamics of gamete fusogen HA…
WebNational Center for Biotechnology Information WebThe structure of Chlamydomonas HAP2 suggests that the cd loop is bipartite, with the two parts split by a small alpha helix (Fe´dry et al., 2024). Given this specific structure/function biological activity observed during the study of HAP2 in Tetrahymena, Arabidopsis, and Chlamydomonas, and the general conservation of the protein WebHAP2 localizes at the fusion site of Chlamydomonas minus gametes, yet Chlamydomonas minus and Plasmodium hap2 male gametes retain the ability, using other, species-limited proteins, to form tight prefusion membrane attachments with their respective gamete partners. Membrane dye experiments show that HAP2 is essential for … daily pisces