Cisplatin hplc method

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August undefined, 2024

WebCisplatin was the first of the platinum-based chemotherapeutic agents and therefore has been extensively studied as an antitumor agent since the late 1960s. Because this agent … WebCisplatin, referred to by chemical name as cis-diamminedichloroplatinum (II), is anticancer, neutral and DNA destroying agent that is square planar platinum (II) complex and contains 2 ligands of chloride in a cis configuration orientation. 1 Cisplatin has been cited as being among the most used cytotoxic anticancer medication due to its ...

Development of an Ultraperformance Liquid …

WebApr 24, 2024 · Doxorubicin hydrochloride. Several methods such as LC-MS, reverse phase HPLC and UV-Visible spectrometry have been reported and validated for quantitative analysis of doxorubicin hydrochloride in bulk and pharmaceutical dosage forms [12,13,14, 22, 23].All these methods require use of solvents, however due to unstable … WebThe HPLC system (LC-20AD model, Shimadzu, Kyoto, Japan) consisted of two pumps (LC 20-AT model), an automatic injector (9SIL-20AD model), an oven (CTO-20AS model), a spectrophotometric detector (SPD-M20A model), and a computer equipped with the analysis software Shimadzu LC. flowers and honey cast

Low-Level (PPB)Determination of Cisplatin in Cleaning Validation …

WebHypoxia can induce chemoresistance, which is a significant clinical obstacle in cancer therapy. Here, we assessed development of hypoxia-induced chemoresistance (HICR) against free versus polymeric cisplatin micelles in a triple negative breast cancer cell line, MDA-MB-231. We then explored two strategies for the modulation of HICR against … WebCisplatin is an anti-tumor agent widely employed in cancer chemotherapy. A specific and selective method for the quantitative determination of cisplatin in human plasma and its … green and white hat guy bleach

Development and Validation of RP-HPLC Method for the …

A Novel Analytical Method of Cisplatin Using the HPLC with a ...

http://www.ijsit.com/admin/ijsit_files/ijsit_1.1.5.pdf WebAug 23, 2012 · Establishing HPLC/ICP-MS quantification methods. For quantification of Cisplatin–DNA adducts, IDA was chosen. For the adduct d(GpG)-Pt(NH 3) 2, Garcia Sar et al. already published a suitable approach. 21 In order to monitor multiple adducts, we have chosen species-unspecific IDA (SU-IDA). With SU-IDA it is possible to study the … flowers and home hot springs arWebSep 1, 2016 · An isocratic reversed-phase high-performance liquid chromatographic method with ultraviolet detection at 254 nm has been developed for the determination of doxorubicin in human plasma. Plasma... flowers and home castle bromwich

"WebAreas covered: In this review, we have discussed about cisplatin and its liposomal formulations, focusing on different preparation methods and analysis approaches such as atomic absorption, mass spectroscopy, UV, electrochemical methods, and emphasizing on HPLC as one of the accurate and specific methods for determination of cisplatin … " - Cisplatin hplc method

Cisplatin hplc method

WebWe successfully determined cisplatin, hydroxo complexes, and OH-dimer by HPLC using a πNAP column. Thus the measurement of cisplatin (cis-diamminedichloro-platinum(II), cis-[PtCl2(NH3)2]) (CDDP) should be done using a πNAP column rather than a C18 column or aminopropylsilyl silica gel column. WebTotal and free cisplatin concentrations were measured using a validated HPLC-UV method. Results: The highest concentration of total and free cisplatin was achieved at the end of the infusion in both regimens. Total cisplatin concentration declined 30 minutes after the end of infusion in both the groups. After 1hour of discontinuing cisplatin ...

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WebApr 1, 2012 · The HPLC method was developed using C 18 ODS Hypersil column of 250 × 4.6 mm id with 5 µm particle size, mobile phase of water : methanol : acetonitrile (40:35:25, v/v/v, pH 3.5) at a flow rate... WebMar 1, 2010 · Cisplatin is an anti-tumor agent widely employed in cancer chemotherapy. A specific and selective method for the quantitative determination of cisplatin in human …

WebMar 24, 2016 · We describe an HPLC–UV method for determining cisplatin levels in liposomal and biological samples, which represents an attractive alternative to the … WebFor cis-diammine (1,1-cyclobutane-dicarboxylato) platinum (CBDCA), normal-phase chromatography on silica with UV detection suffices down to levels of 10 μM in urine or plasma ultrafiltrates. Quantitation below 10 μM needs HPLC fraction collection followed by flameless atomic absorption spectrophotometry (FAAS; 0.5 μM detectable).

WebDec 1, 2024 · Tezcan et al. [70] described a HPLC-PDA method for monitoring the plasma concentration of free cisplatin in different types of cancer. Deproteinization with cold MeOH was used for sample extraction of cisplatin and NiCI 2 .6.H 2 O as IS. WebA high-performance liquid chromatographic (HPLC) method is described for the determination of residual levels of cisplatin from extracts of surfaces with very low …

WebMaterials and Methods Chemicals. Reduced glutathione (GSH), glutathione oxidized, and g-glutamyl glutamate, superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase, solvents of analytical grade (methanol and glacial acetic acid) used for HPLC, thioctic acid (a-lipoic acid, oxidized form) and cisplatin were purchased ...

WebApr 14, 2024 · Among the extracellular vesicles, apoptotic bodies (ABs) are only formed during the apoptosis and perform a relevant role in the pathogenesis of different diseases. Recently, it has been demonstrated that ABs from human renal proximal tubular HK-2 cells, either induced by cisplatin or by UV light, can lead to further apoptotic death in … flowers and honeyWebNov 1, 2008 · Purpose: The purpose of this study was to develop and validate HPLC-UV method for the determination of the cisplatin, based … flowers and home bryant arWebMar 1, 2024 · Cisplatin is a platinum containing compound used for the treatment of different types of cancers. Quantitative determination of cisplatin has been carried out … flowers and honey julie tennerhttp://www.ijsit.com/admin/ijsit_files/ijsit_1.1.5.pdf green and white hawaiian shirtWebThe method was successfully applied for routine analysis of Oxaliplatin tablet dosage form and bulk drug. Key Words:Oxaliplatin, RP-HPLC, UV detection, recovery, precise, 240nm INTRODUCTION Oxaliplatin is a platinum-based antineoplastic agent that is used in cancer chemotherapy. oxaliplatin is the platinum-containing complex similar to cisplatin. green and white hatsWebSep 1, 2024 · Application of a newly developed HPLC method to evaluate the drug release of co-loaded 5-fluorouracil and cisplatin films. Abstract The combination of 5-fluorouracil (5-FU) and cisplatin (Cis) has been used for the treatment of many cancers. flowers and honey filmWebAug 15, 2007 · The HPLC method was developed for individual as well as simultaneous determination of two drugs namely carboplatin and paclitaxel in the presence of polymer … flowers and home in bryant ar