Click-it edu cell proliferation kit alexa 594
WebParaffin sections (5 µm thick) were processed to detect Edu-positive cells, and cell proliferation was analysed using the Click-iT Plus Alexa Fluor Picolyl Azide Toolkit (Thermo Fisher Scientific). For analysis of cellular apoptosis, TUNEL staining was performed using the In situ Apoptosis Detection Kit (Takara Bio) according to the ... WebMar 9, 2015 · In contrast, the EdU cell proliferation kit is compatible with cell cycle dyes (Figure 2). The EdU assay can also be multiplexed with antibodies against surface and intracellular markers. However, some reagents or antibody conjugates may not be compatible with the Click-iT® EdU detection reaction and may need some additional …
Click-it edu cell proliferation kit alexa 594
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WebThe kit contains all of the components needed to label and detect the incorporated EdU as well as perform cell cycle analysis on samples from adherent cells. For cell cycle analysis, the kit includes a blue fluorescent Hoechst 33342 dye. The kit contains sufficient reagents for labeling 50 18x18 coverslips using 500 µL of reaction buffer per test. WebThe Click-&-Go ® EdU 594 Flow Cytometry Assay Kit is a superior alternative to the traditional cell proliferation assays that use radioactive nucleosides, i.e., 3H-thymidine or antibody-based detection of the nucleoside analog bromodeoxyuridine (BrdU). EdU (5-ethynyl-2′-deoxyuridine) is a nucleoside analog of thymidine with a terminal alkyne group …
Web(C) In contrast, Erk2-GFP A375 cells processed using the reagents and fixation/detection protocol provided in the Click-iT Plus EdU Alexa Fluor 594 Proliferation Assay Kit retained their GFP signal (green), and the EdU-based detection of proliferation was very bright (red, 8 msec exposure). Both cell samples were treated with Hoechst 33342 ... WebBCK488-IV-IM-S 50 mg EdU-Click Kit 6-FAM Azide ... -1M-1 Alexa 488, DyLight488, FluorX, ATTO488 5-TAMRA-PEG3- ... designed in a sense that you combine one of our three baseclick EdU cell proliferation kits such as Imaging kit (BCK-IV-IM), flow cytometry kit (BCK-IV-FC) and our high throughput ...
WebFigure 1. Fluorescence signal from Pacific Blue™, Alexa Fluor® 488, and Alexa Fluor® 647 Click-iT® EdU Flow Cytometry Assay Kits. Jurkat (human T-cell leukemia) cells were … WebIn contrast, the Click-iT™ Plus EdU cell proliferation kit is compatible with cell cycle dyes, R-PE, R-PE tandems, and fluorescent proteins such as GFP, RFP, and mCherry. See page 5 for examples of Click-iT ™ Plus EdU Alexa Fluor 488 multiplexed with R-PE, Click-iT™ Plus EdU Alexa Fluor 350 multiplexed with Pacific Orange™
WebClick-&-Go® Plus EdU Cell Proliferation Assays Click-&-Go® Plus EdU Flow Cytometry Kits Click-&-Go® EdU Flow Cytometry Kits. ... 594 Laser line Tx Red Common filter set. Catalog #: 1328 -+ $ 249.00. Add to cart. Click ...
WebApr 12, 2024 · On day 5, 10 μM EdU was added to the cells and incubated at 37°C for 2 hours. EdU incorporation was measured using a Click-it Plus EdU Alexa Fluor 594 Imaging Kit (Invitrogen) in accordance with the manufacturer’s instructions. Images were captured on a Zeiss Observer microscope. loremaster of kalimdorWebEdU Cell Proliferation Assay EdU-488 Catalog No. 17-10525 EdU-555 Catalog No. 17-10526 EdU-594 Catalog No. 17-10527 EdU-647 Catalog No. 17-10528 100 reactions FOR RESEARCH USE ONLY Not for use in diagnostic procedures. USA & Canada Phone: +1(800) 645-5476 In Europe, please contact Customer Service: France: 0825.045.645; … lore master wiz101WebCellTrace CFSE Cell Proliferation Kit ... ERK2 A375 GFP expressing cells labeled with Click-iT Plus EdU Alexa Fluor 647 Imaging Kit (pink), Proliferating cells have pink nuclei. Non-proliferating cells have blue nuclei. ... Alexa Fluor 555 Alexa Fluor 594 Alexa Fluor 647; Excitation/Emission (in nm) 350/461: 495/519: 555/565: 590/615: 650/670 ... horizons by marriott branson moWebPrepare stock solutions. Add 2 mL DMSO (Component C) or an aqueous solution to Component A to make a 10 mM EdU stock solution. Store at –20°C. Make 1X Click-iT® EdU reaction buffer by adding 36 mL of deionized water to Component D. Store at 2–8° C. Make Alexa Fluor® azide by adding 70 μL DMSO (Component C) to Component B, then … loremaster of voss swtorWebThe Click-iT EdU Cell Proliferation Kit for Imaging provides a superior alternative to BrdU assays for measuring cell proliferation. EdU (5-ethynyl-2'-deoxyuridine) is a nucleoside … Dual parameter plot of Click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay … Click-iT EdU Alexa Fluor 594 Imaging Kit provides a superior alternative to BrdU … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes lore mass effectWebArticle Title: Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation. Journal: Cell Stem Cell. ... Click It Edu Alexa Fluor 594 Image Kit, supplied by … lorem cityWebBeyoClick™ EdU细胞增殖检测试剂盒(TMB法)(BeyoClick™ EdU Cell Proliferation Kit with TMB)，是一种基于DNA合成过程中胸腺嘧啶脱氧核苷(thymidine)类似物EdU(5-ethynyl-2' -deoxyuridine)的掺入，并通过随后的点击反应(Click reaction)使EdU被生物素(Biotin)所标记，然后加入的辣根过氧化物酶标记链霉亲和素(HRP-Streptavidin)与生物素 ... loremaster of korriban