WebHoechst 33342 dye, a cell-permeant nucleic acid stain that is selective for DNA and spectrally similar to DAPI, is UV excitable and emits blue fluorescence when bound to DNA. This dye does not interfere with GFP fluorescence and is retained after fixation … Probes for Lysosomes, Peroxisomes and Yeast Vacuoles—Section 12.3; Probes … LysoTracker Deep Red is a cell-permeable, non-fixable, red fluorescent dye that …

IDH3γ functions as a redox switch regulating mitochondrial energy ...

WebNov 24, 2024 · We then tested the respective compatibilities of these dyes with fluorescent reporter lines (Figure 2).In order to demonstrate the usefulness of Direct Red 23, we combined it with a phloem-specific … WebTriton X-100 is probably the most commonly used permeabilization agent for immunofluorescent staining. This detergent efficiently dissolves cellular membranes without disturbing protein-protein interactions (Figure 1). Triton is usually used at concentrations ranging from 0.1 to 1% for permeabilization. the outsiders book chapters

Fluorescence Labeling and Detection Strategies in Cellular …

WebApr 12, 2024 · Therefore, fluorescent–photoacoustic dual-modality detection for the viscosity of peroxisome was achieved by PV-1. Further cell experiments proved that the probe exhibited good biocompatibility and could accurately accumulate in peroxisome ( Figure 5 D) for the fluorescence imaging of viscosity changes caused by lipid metabolism … WebDec 3, 2024 · A wide range of commercially available dyes and reagents have been created to visualize core cellular structures and organelles [3, 4], including the plasma membrane, nucleus, endoplasmic reticulum, Golgi apparatus, mitochondria, lysosomes, peroxisomes and cytoskeletal network [5,6,7,8] (Fig. 1). However, the extensive breadth of these … WebDec 28, 2001 · To measure the pH of intact peroxisomes in situ, we used the peroxisome-specific carboxyl-terminal targeting sequence, SKL, to deliver a pH-sensitive mutant of the green fluorescent protein (pHluorin-SKL) selectively into peroxisomes. Proper targeting was verified by colocalization with the peroxisomal marker catalase. the outsiders book character descriptions