WebPrimary analysis of raw data, including sequence alignment to rCRS and variant calling, was performed with the Ion Torrent™ Suite 5.10.1 (Thermo Fisher Scientific, Waltham, MA, USA) software and HID Genotyper 2.2 and Coverage Analysis (v5.10.0.3) plugins. Web7 aug. 2014 · The Ion Chef is Life Techs new sample prep machine for automated library and size selection. The 1 hour is for the library amp step, instead f the 5 hour step now. …
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Web14 apr. 2024 · The Ion Torrent S5 system (Thermo Fisher Scientific Inc.) enabled sequencing workflows. The sequencing reads were filtered by mapping quality (>10) and read length (70–130 bp) using SAMtools [ 11 ]. The GRCh37 human genome assembly was used as a reference. Web9 apr. 2024 · Sequencing was performed with the Ion Torrent™ Ion S5™ system. Four genes were detected with highest mutation incidence: KRAS (43.9% of all cases), followed by ALK (36.6%), TP53 (31.7%), and PIK3CA (29.3%). Seven patients had co-occurring KRAS + TP53 (6/41, 14.6%) or KRAS + PIK3CA (7/41, 17.1%) mutations. on with mario daily podcast
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WebTransfer a Planned Run to a Ion Torrent Server with Ion Mesh; Track Ion Chef Instrument flexible workflows for Ion 550 chips with Ion Mesh; Data backup and restore locations. … WebAMP, or Anchored Multiplex PCR, is a rapid and scalable method to generate target-enriched libraries for NGS. AMP technology can be used for applications in targeted … WebThe Ion 550 Kit enables robust and automated template preparation and sequencing of up to 200 base-read libraries using the Ion Chef System and the Ion GeneStudio S5 Plus, Ion GeneStudio S5 Prime or Ion S5 XL sequencing systems. The Ion 550 Kit contains pre-packaged template and sequencing reagent cartridges with integrated sample tracking, ... iotx chart