Thp1 gene
WebNov 1, 2014 · THP-1 monocytes maximally expressed IL-1β, IL-6, IL-8, IL-10 and TNF-α genes after 3 h of LPS stimulation, while gene expression of THP-1 macrophages appeared to be … WebElevated CCL2 gene (A) and secreted protein (B) expression is shown in THP-1 monocytic cells co-stimulated with IFN-γ and LPS compared to cells treated with IFN-γ and LPS alone. Primary monocytes ( C ) also show increased CCL2 secreted protein expression after co-stimulation with IFN-γ and LPS compared to stimulation with either IFN-γ or LPS.
Thp1 gene
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WebAssay Overview. This assay is to provide THP-1-based In Vitro Reporter Gene (Luciferase) Assay to accelerate our client's oncology projects. The assay will be customized … WebApr 5, 2024 · The results showed that the mutant allele of rs11568821 (A) and rs41386349 (T) polymorphisms is associated with an increase in HTLV-1 infection significantly, but there was no significant relationship between PVL and polymorphisms. About 90% of people infected with Human T lymphotropic virus type-1 (HTLV-1) virus are asymptomatic, so it …
WebGAS5, MMP2, and MMP9 Gene Expression in the THP1 Cell Line. The THP1 human monocytic cell line was chosen as the cellular model to confirm the involvement of GAS5 in the regulation of MMP2 and MMP9 ... aggravates inflammatory response and MMP expression in THP-1 macrophages by acting like a sponge for miR-221. Exp. Cell Res. … Web1 day ago · A mutation in this gene results in wasting of carnitine by the kidney, ... and also ICAM-1 (intercellular adhesion molecule-1) in one study, 138,139 promoting monocytic THP-1 cell adhesion to endothelial cells, but this was not confirmed in another study. IxS may induce vascular smooth muscle cell (VSMC) dysfunction by promoting proliferation, ...
WebLevels of IL-6, −8, −10, TNF-α, MMP-9 and TIMP-1 in LPS-exposed H292 and THP-1 cells. LPS is a potent proinflammatory activator of monocytes and macrophages in vivo and in vitro … WebGene knockdown was carried out using Trilencer-27 siRNA pool against Mincle or a scrambled siRNA control (OriGene Technologies, MD). Briefly, prior to transfection …
WebSupplementary Figure 4. The expression of G3BP1 and ZYG11B in stable gene knockdown cells. Related to Figure 7. Specific RNAi to ZYG11B (Zi#1) (A) or pCDH-CMV-ZYG11B (B) was transduced into G3BP1 knockdown THP-1 cells by retroviral-mediated gene transfer. The expression of G3BP1 and ZYG11B at the mRNA level was analyzed by qPCR.
WebThe process usually begins with the identification of a target nucleic acid whose expression is to be modulated. As used herein, the terms "target nucleic acid" and "nucleic acid encoding a target gene" encompass DNA encoding a selected target gene, RNA (including pre-mRNA and mRNA) transcribed from such DNA, and also cDNA derived from such RNA. good for health in spanishWebInside vitro select of differing broadened functions are useful since human monocyte-derived macrophages are short-lived, limited and vary from donor on donor. Published protocols using the promonocytic cell line THP-1 have tended to output in cells that closely resemble classically-activated macrophage … health theme wordpressWebCYP2E1, as a member of the cytochrome P450s (CYPs) super-family, is in charge of six percent drug metabolism involving a diversity of drugs distinct in structures and chemical properties, such as alcohols, monocyclic compounds (e.g., acetaminophen, benzene, p-nitrophenol), bicyclic heterocycles (e.g., coumarin, caffeine) and even fatty acids. The … health theoryWebCCAAT/enhancer binding protein β (C/EBPβ) is a transcription factor that regulates the expression of important proinflammatory genes in microglia. Mice deficient for C/EBPβ show protection against excitotoxic and ischemic CNS damage, but the involvement in this neuroprotective effect of the various C/EBPβ-expressing cell types is not solved. health themes for each month 2022WebJun 21, 2024 · THP-1 iCas9 cells were transduced with a genome-wide sgRNA library containing 6 guides per gene and 500 non-targeting controls (18,187 genes, ~ 6 sgRNA … health theme for preschoolWebCytogenetic and molecular analysis of the acute monocytic leukemia cell line THP-1 with an MLL-AF9 translocation. Genes Chromosomes Cancer 29:333-338(2000) DOI= … health theoriesWebthe migration of another THP1 monocyte cell line that stably expresses GFP for visualization.41 The top chambers of the transwells were filled with equal numbers of a … health themes by month